Composite nanoclay-hydroxyapatite-polymer fiber scaffolds for bone tissue engineering manufactured using pressurized gyration

A novel fabrication of polymer composite fibers using polycaprolactone (PCL), montmorillonite nanoclay (MMT-Clay), and nano-hydroxyapatite-clay (HAP MMT-Clay) is reported for bone tissue engineering applications. Using a pressurized gyration (PG) setup, (PCL) incorporated with in situ mineralized HAP MMT-Clay were investigated. the method, we able to successfully manufacture HAP-nanoclay-PCL fibers. Further, 3D scaffolds made prepared enhance growth, cell viability, proliferation. The results demonstrated that fiber are biocompatible, cells thrive differentiate on scaffolds. significant increase osteogenic differentiation, ECM formation, collagen formation was observed PCL compared behaviors intracellular ALP levels increased scaffold, indicating enhanced differentiation MSCs. This work shows promising outlook future manufacturable as